Abstract We investigated the Biogran on bone repair and metabolism at several time-intervals upon grafting into rat tibia artificial defects. The biomaterial was thoroughly characterized in vitro, and its dissolution behavior upon immersion was assessed in simulated body fluid (SBF) solution for 1, 3, 7, 14, and 30 days. Biogran was also assessed by in vitro hydroxyapatite formation in SBF solution, which is a marker for bioactive behavior. In vivo, distal and proximal bone defects were performed in the Wistar rat's right tibia and filled according to the experimental groups: I) negative control, no filling; II) positive control, 10 mg of autogenous bone; and III) 10 mg of Biogran. Animals were euthanized at 1, 2, 3, 4, 7, and 10 weeks. Bone neoformation was analyzed using histomorphometry (proximal defect), and local levels of bone morphogenetic protein 2 (BMP-2) were measured using the ELISA assay (distal defect). In vitro, the Biogran sample showed a fast dissolution rate within the first 7 days, parallel to the formation of the hydroxyapatite layer. In vivo, the sample was progressively resorbed at a higher rate within the first month until it became almost absent at week 10th. The sample presented similar or higher bone neoformation concerning the autogenous bone. BMP-2 levels were sustained in the Biogran group (around 200 pg/mg) and detected until the last experimental time with a significant difference compared with the controls. Results suggest Biogran is a candidate for hard tissue engineering even in highly active bone remodeling sites.

Resumo Este estudo avaliou o efeito da terapia fotodinâmica (PDT) em canais radiculares infectados com E. faecalis. Vinte e um dentes humanos extraídos foram selecionados, e 18 foram infectados por E. faecalis por 60 dias. As estratégias antimicrobianas testadas foram: G1. Preparo do canal radicular (PCR) com instrumentos rotatórios de NiTi, NaOCl 2,5% e irrigação final com EDTA 17%, seguido de PDT com fotossensibilizador azul de metileno e laser diodo de baixa potência; G2. PCR usando limas de aço inoxidável e os mesmos protocolos de irrigação e PDT do G1; G3. Protocolo de PCR similar que G1 sem PDT; G4. Somente irrigação com NaOCl 2,5%; G5. Protocolo similar ao G1, sem PCR; G6. Controle negativo; G7. Controle positivo. Amostras para exames microbiológicos foram coletadas inicialmente (S1), após PCR (S2) e após PDT (S3). Na sequência, as raízes foram seccionadas e preparadas para análise em microscopia eletrônica de varredura (MEV). O crescimento bacteriano foi analisado de acordo com a turbidez do meio de cultura seguida pela densidade óptica espectrofotométrica (nm). O efeito da PDT na estrutura dentinária foi avaliado em aumentos de 1.600X e 5.000X, e descrito qualitativamente. O teste de Wilcoxon foi utilizado para as comparações dos mesmos espécimes e o teste de Mann-Whitney para as comparações entre os grupos ((=5%). Bactérias foram encontradas em todos os grupos experimentais, e em todas as coletas microbiológicas (S1, S2 e S3). A densidade óptica dos meios de cultura foi menor em S2 do que em S1 de G1, 2, 3 e 4 (p>0,05). Após a PDT (S3) em G1 e 2, houve redução adicional na densidade óptica do meio de cultura de 90,0% e 92,0%, respectivamente (p>0,05). No Grupo 5, a análise dos meios de cultura em S2 revelou um aumento de 3,2% na densidade óptica em comparação com S1(p>0,05). Nas imagens de MEV do G1, 2 e 5 foram evidenciadas dentina com áreas de fusão e recristalização. O PDT utilizado após preparo do canal radicular com sistema rotatório ou manual, associado ao NaOCl 2,5%, não foi capaz de eliminar completamente o E. faecalis em biofilme maduro presente no canal radicular.

Abstract This study evaluated the effect of photodynamic therapy (PDT) on infected root canals. Twenty-one human teeth were selected, and 18 were infected by E. faecalis for 60 days. The antimicrobial strategies tested were: G1. Root canal preparation (RCP) using Niquel-Titanium (NiTi) rotary instruments, 2.5% NaOCl, and final irrigation with 17% EDTA, followed by PDT with methylene blue photosensitizer and laser diode low power; G2. RCP using stainless steel files and the same irrigation and PDT protocols as G1; G3. Same RCP protocol as G1 without PDT; G4. Only irrigation with 2.5% NaOCl; G5. Same PDT protocol as G1 without RCP; G6. Negative control; G7. Positive control. Samples for microbiological tests were collected initially (S1), after RCP (S2), and after PDT (S3). Subsequently, the roots were sectioned and prepared for Scanning Electron Microscopy (SEM) analysis. Bacterial growth was analyzed according to the turbidity of the culture medium, followed by spectrophotometric optical density (nm). The effect of PDT on the dentinal structure was evaluated at magnifications 1,600X and 5,000X and described qualitatively. The Wilcoxon test was used for the comparisons from the same specimens, and the Mann-Whitney test was used to compare groups ((=5%). Bacteria were found in all experimental groups’ microbiological samples (S1, S2 and S3). The optical density of culture media was lower in S2 than in S1 of G1, 2, 3, and 4 (p> 0.05). After PDT (S3) in G1 and 2, there was an additional reduction in optical density of the culture medium, respectively (p>0.05). In Group 5, the analysis of culture media at S2 revealed an increase in optical density compared to S1(p>0.05). In SEM images of G1, 2, and 5, dentin with melting and recrystallization areas were evidenced. After preparation of the root canal with the rotary system or manually associated with 2.5% NaOCl, PDT was not able to completely eliminate E. faecalis present in the root canal.

SUMMARY OBJECTIVE: The aim of this study was to assess the inter-reliability of the Braden scale and its subscales for different patients assisted in the intensive care unit. We hypothesized that the Braden scale has low reliability in different populations. METHODS: This reliability study involved the Braden scale in intensive care unit of a hospital. A total of 200 patients were admitted to the intensive care unit in four different groups: neurological patients, sepsis, elderly, and adults affected by trauma. The Braden scale is a tool composed of six subscales for patient assessment: sensory perception, humidity, activity, mobility, nutrition, and friction. The total score was also calculated. The Braden scale was applied by two different nurses with an interval of 20–30 min between applications. RESULTS: For all populations, kappa values considered unsuitable were observed for most categories of the Braden scale, ranging from 0.06–0.25. Only for the total Braden scale score was moderate reliability identified in all groups evaluated, with intraclass correlation coefficient values ranging from 0.48–0.75. CONCLUSIONS: Braden scale is not a reliable tool to be used in the intensive care unit, and we do not recommend the use of this scale to assess the risk of developing pressure injury.

Abstract: This study measured the thickness of cementum/dentin in the danger zone of the mandibular molars after root canal preparation using novel cone-beam computed tomography (CBCT) software. Eighty-four teeth were distributed into four groups: ProTaper Next, BioRace, Reciproc Blue, and WaveOne Gold. E-Vol DX® CBCT software was used to measure initial and final remaining cementum-dentin thicknesses after root canal preparation of the mesial root of mandibular molars at 1 and 3 mm from the furcation. The Kolmogorov-Smirnov test was used to test variable symmetry. The variables were described as mean and standard deviations, compared among the groups using analysis of variance (ANOVA), and within the groups using the Student t test. A generalized estimating equation model was used to compare the variation before and after root canal preparation. The level of significance was set at 5%. Differences between mean initial and final thicknesses of the mesiobuccal (MB) and mesiolingual (ML) canals were not statistically significant. The mean initial thickness was 3 mm (0.900 mm ± 0.191), considering that a mean lower than 1 mm (1.035 mm ± 0.184) indicates the danger zone. Although cementum/dentin is thinner at 3 mm from the furcation (0.715±0.186) after root canal preparation, the greatest amount of dentin removed was found at 1 mm (0.734 ± 0.191). The cementum-dentin remaining after preparation was thicker than 0.715 mm in root canals prepared using #35 (WaveOne Gold®) and #40 (ProTaper Next®, BioRace® and Reciproc Blue®) instruments. This confirms the safety of canal preparation in the danger zone using these systems.

RESUMO O artigo examina as relações entre os sujeitos da aprendizagem, tanto o estudante quanto o/a professor/a, e a mobilização do conceito de democracia na Base Nacional Comum Curricular (BNCC) correspondente à etapa do Ensino Médio, com destaque para a área de Ciências Humanas e Sociais Aplicadas na versão final de 2018. A partir das circunstâncias históricas de produção dessa versão final da BNCC e da delimitação teórica dos dilemas que afetam as subjetividades contemporâneas, é analisado o modo pelo qual o conceito de democracia figura no texto da base curricular mencionada. Constata-se que a democracia é situada como regulador das aspirações ético-políticas de estudantes, sob o viés, no entanto, de horizonte de estabilidade política, desprovida de historicidade, esvaziando assim a agência e o enfrentamento da crise contemporânea pelos sujeitos da aprendizagem.

ABSTRACT The article examines the relationships between the subjects of learning, both the student and the teacher, and the mobilization of the concept of democracy, in the National Common Curricular Base, stage of High School, highlighting the area of Applied Human and Social Sciences, final version of 2018. The way in which the concept of democracy appears in the current curriculum base text is analyzed, based on the historical circumstances of the production of this final version of this curriculum and, also, based on the theoretical delimitation of the dilemmas that affect contemporary subjectivities. The main conclusion is that democracy is situated as a regulator of the ethical-political aspirations of students, under the bias, however, of a horizon of political stability, devoid of historicity, thus emptying the agency and the confrontation of the contemporary crisis by the subjects of learning.

Resumo Este estudo teve como objetivo comparar os valores da pressão intraocular (PIO) por diferentes tonômetros e avaliadores (veterinário oftalmologista especialista e veterinário não especialista). Para isso, foram utilizados 30 coelhos, em todos (n = 60 olhos), a PIO foi medida inicialmente com um tonômetro de rebote (model TD - 8000 portable, Apramed Indústria e Comércio de Equipamentos Médicos Ltda) e, posteriormente, com um tonômetro de aplanação (portable model Tono-Pen AviaTM®, Reichert Technologies®, USA). Com os dois aparelhos, as medidas em mmHg foram realizadas na região central das córneas, sempre no mesmo período, por um profissional oftalmologista veterinário (especialista) e um profissional veterinário (não especialista). Os dados foram comparados estatisticamente por meio do teste de análise de variância simples. Com o tonômetro de rebote, a PIO variou de 7 a 14 mmHg quando medida por ambos os avaliadores; enquanto com o tonômetro de aplanação, de 9 a 15 mmHg pelo especilista e de 8 a 16 mmHg pelo não especialista. Nos olhos direitos, a PIO medida pelo tonômetro de aplanação pelo avaliador não experiente foi estatisticamente inferior aos valores do especialista; ainda, os resultados dos dois avaliadores foram maiores nestes mesmos olhos quando comparados com os do tonômetro de rebote. Nos olhos esquerdos, a PIO medida pelo tonômetro de aplanação pelo avaliador não experiente foi estatisticamente superior aos valores do especialista com o tonômetro de rebote. Assim, foi possível inferir que, independente da experiência na área, o tonômetro de aplanação indicou maiores valores médios de PIO em ambos os olhos e, em relação aos avaliadores, as médias das medidas realizadas pelos especialistas foram maiores em relação ao não especialista.

Abstract This study aimed to compare values of intraocular pressure (IOP) by different tonometers and evaluators (veterinary ophthalmologist specialist and veterinary not a specialist). For this, 30 rabbits were used, and in all (n = 60 eyes), the IOP was initially measured with a rebound tonometer (model TD - 8000 portable, Apramed Indústria e Comércio de Equipamentos Médicos Ltda) and, subsequently, with an applanation tonometer (portable model Tono-Pen AviaTM®, Reichert Technologies®, USA). With the two devices, the measurements in mmHg were performed in the central region of the corneas, always performed in the same period, by a professional veterinary ophthalmologist (specialist) and a professional veterinary (not a specialist). Data were statistically compared using the simple analysis of variance test. With the rebound tonometer, IOP ranged from 7 to 14 mmHg when measured by both evaluators; while with the applanation tonometer, from 9 to 15 mmHg by the specialist and from 8 to 16 mmHg by the non-specialist. In the right eyes, the IOP measured by the applanation tonometer by the non-experienced evaluator was statistically lower than the specialist's values; yet, the results of the two evaluators were higher in these same eyes when compared with those of the rebound tonometer. In the left eyes, the IOP measured by the applanation tonometer by the non-experienced evaluator was statistically higher than the specialist's values with the rebound tonometer. Thus, it was possible to infer that, regardless of experience in the area, the applanation tonometer indicated higher mean values of IOP in both eyes and, about the evaluators, the means of the measurements performed by the specialist were higher compared to the non-professional specialist.

Resumo A Curcuma longa L., planta conhecida popularmente como açafrão, tem sido amplamente estudada por suas diversas propriedades terapêuticas, incluindo a ação antineoplásica. O extrato etanólico da planta contém diversos compostos fenólicos, com destaque para a curcumina. O osteossarcoma é um tumor ósseo predominante em cães e humanos, caracterizado por apresentar alto potencial metastático e prognóstico desfavorável. Procurou-se investigar os efeitos de diferentes concentrações de curcumina do extrato etanólico de açafrão sobre células de osteossarcoma canino de cultura estabelecida. As células foram cultivadas e submetidas ao tratamento com extrato com diferentes concentrações de curcumina (0, 10 μM, 20 μM, 50 μM, 100 μM e 1000 μM) e tempos de exposição (24h, 48h e 72h) pelo EEA. Inicialmente, foram realizados: técnica de redução do tetrazólio (MTT) e cálculo da IC50. Posteriormente, após o tratamento com o extrato, realizou-se o ensaio de imunocitoquímica para verificar a expressão de p53 mutada e estudar o potencial proliferativo das células malignas; Bcl-2, com intuito de averiguar o estímulo de via antiapoptótica; e o marcador Ki-67, que sinaliza aumento no grau de malignidade. O extrato promoveu proliferação de células de osteossarcoma canino, com incremento de até 3819,74% na concentração de 50μM de curcumina. O composto também alterou a expressão das proteínas p53 mutante e Ki-67 significativamente, mas não alterou a expressão de Bcl-2, mostrando que não induziu a via antiapoptótica mediada por esta. Estes resultados demonstram que o extrato etanólico do açafrão apresenta potencial proliferativo sobre células de osteossarcoma canino, sugerindo a necessidade de conscientização e conhecimento dos reais efeitos de determinados compostos naturais, considerados seguros ao serem utilizados como tratamento de diversas enfermidades.

Abstract Curcuma longa L., also known as turmeric, has been widely studied for its various therapeutic properties, including antineoplastic action. The ethanolic extract of the plant contains several phenolic compounds, especially curcumin. Osteosarcoma is a predominant bone tumor in dogs and humans, characterized by high metastatic potential and an unfavorable prognosis. The aim of this study was to investigate the effects of turmeric ethanol extract on canine osteosarcoma cells from established culture. The cells were cultured and treated with different curcumin concentrations (0, 10 μM, 20 μM, 50 μM, 100 μM, and 1000 μM) and exposure times (24h, 48h, and 72h). We first performed tetrazolium reduction technique (MTT) assay and calculated IC50. An immunocytochemistry assay was performed after extract treatment to verify the expression of mutated p53 and therefore study the proliferative potential of malignant cells; Bcl-2 and Ki-67 were used to assess apoptosis and the degree of malignancy, respectively. The extract enhanced the proliferation of canine osteosarcoma cells, reaching 3,819.74% at 50 μM of curcumin. The extract also significantly altered the expression of mutated p53 and Ki-67 proteins but not that of Bcl-2, suggesting that it did not induce this antiapoptotic pathway. Overall, these results are prerequisite to better understanding how natural compounds such as turmeric ethanolic extract affect cell proliferation and could be used to treat various diseases.

Abstract wastewater treatment (WT) is of major importance on modern cities, removing wastewater pollutants resultant from anthropogenic activities. The unique abilities of microbes to degrade organic matter, remove nutrients and transform toxic compounds into harmless products make them essential players in waste treatment. The microbial diversity determines the metabolic pathways that may occur in WT and quality of treated wastewater. Therefore, understanding WT microbial community structure, distribution, and metabolic functioning is essential for development and optimization of efficient microbial engineering systems. Since cultivation methods can only detect a small fraction of the microbial diversity, the use of culture-independent molecular methods has circumvented this issue, allowing unprecedented access to genes and genomes used for microbial composition and function evaluation. Traditional approaches like RAPD, DGGE, ARDRA, RISA, SSCP, T-RFLP, and FISH and modern approaches like microarray, qPCR, and metagenomics are essential techniques for identifying and depicting the total microbial community structure and their interaction with environmental and biotic factors. Thus, this review describes traditional and state of the art molecular techniques which provide insights into phylogenetic and functional activities of microbial assemblages in a WT system.

Abstract INTRODUCTION: This epidemiological household survey aimed to estimate the prevalence of the current and past SARS-CoV-2 infections in Ribeirão Preto, a municipality of southeast Brazil. METHODS: The survey was conducted in two phases using a clustered sampling scheme. The first phase spanned May 1-3 and involved 709 participants. The second phase spanned June 11-14, 2020, and involved 646 participants. RESULTS: During the first phase, RT-PCR performed on nasopharyngeal swabs was positive at 0.14%. The serological tests were positive in 1.27% of the patients during the first phase and 2.79% during the second phase. People living in households with more than five members had a prevalence of 10.83% (95%CI: 1.58-74.27) higher than those living alone or with someone other. Considering the proportion of the positive serological test results with sex and age adjustments, approximately 2.37% (95%CI: 1.32-3.42) of the population had been cumulatively infected by mid-June 2020, which is equivalent to 16,670 people (95%CI: 9,267-24,074). Considering that 68 deaths from the disease in the residents of the city had been confirmed as at the date of the second phase of the survey, the infection fatality rate was estimated to be 0.41% (95%CI: 0.28-0.73). Our results suggest that approximately 88% of the cases of SARS-CoV-2 infection at the time of the survey were not reported to the local epidemiological surveillance service. CONCLUSIONS: The findings of this study provide in-depth knowledge of the COVID-19 pandemic in Brazil and are helpful for the preventive and decision-making policies of public managers.

Resumo Objetivo identificar diagnósticos de enfermagem da Taxonomia da NANDA-I em idosos institucionalizados. Método estudo descritivo, conduzido com 116 idosos de uma instituição de longa permanência, realizado a partir da identificação de diagnósticos de enfermagem da Taxonomia da NANDA-I em um instrumento fundamentado no modelo teórico das Necessidades Humanas Básicas e validação por especialistas com suporte no raciocínio diagnóstico de Risner. Resultados identificou-se 1.555 títulos de diagnósticos de enfermagem para os 116 idosos, com uma média de 13,4 diagnósticos por idoso. Após exclusão de repetições foram obtidos 39 títulos distintos de diagnósticos. Entre eles, 26 (66,7%) são títulos de diagnósticos reais, 13 (33,3%) títulos de diagnósticos de risco e estão classificados em 8 dos 13 domínios da taxonomia da NANDA-I. Os diagnósticos de enfermagem mais frequentes foram: risco de quedas (94,8%); síndrome do idoso frágil (91,3%) e processos familiares disfuncionais (90,5%). Conclusão e implicações para a prática o perfil de diagnósticos de enfermagem identificado pode contribuir para o incremento de indicadores sensíveis à prática de enfermagem, com o planejamento e implementação de medidas assistenciais direcionadas as reais necessidades dos idosos institucionalizados impactando na autonomia, independência e consequentemente na qualidade de vida.

Abstract Objective To identify nursing diagnoses of the NANDA-I Taxonomy in institutionalized older adults. Method A descriptive study, conducted with 116 older adults from a long-term institution, carried out based on the identification of nursing diagnoses of the NANDA-I Taxonomy in an instrument based on the theoretical model of Basic Human Needs and validation by experts with support in Risner's diagnosis reasoning. Results 1,555 nursing diagnosis titles were indicated for the 116 older adults, with a mean of 13.4 diagnoses per individual. After excluding repetitions, 39 different diagnosis titles were obtained. Among them, 26 (66.7%) are titles of real diagnoses, 13 (33.3%) are titles of risk diagnoses and are classified in 8 of the 13 domains of the NANDA-I taxonomy. The most frequent nursing diagnoses were the following: risk for falls (94.8%); frail elderly syndrome (91.3%), and dysfunctional family processes (90.5%). Conclusion and implications for the practice The profile of the nursing diagnoses identified can contribute to the increase of sensitive indicators to the nursing practice, with the planning and implementation of care measures directed to the real needs of institutionalized older adults, impacting on autonomy, independence and, consequently, on quality of life.

Resumen Objetivo identificar diagnósticos de enfermería de la taxonomía NANDA-I en ancianos institucionalizados. Método estudio descriptivo, realizado con 116 personas mayores de una residencia gerontológica, a partir de la identificación de diagnósticos de enfermería de la taxonomía NANDA-I en un instrumento basado en el modelo teórico de Necesidades Humanas Básicas y validado por especialistas con apoyo en razonamiento Diagnóstico de Risner. Resultados se indicaron 1.555 títulos de diagnóstico de enfermería para los 116 p edad adultos mayores, con un promedio de 13.4 diagnósticos por adulto mayor. Excluidas las repeticiones, se obtuvieron 39 títulos de diagnóstico diferentes. Entre ellos, 26 (66.7%) son títulos de diagnósticos reales, 13 (33.3%) son títulos de diagnósticos de riesgo, y se clasifican en 8 de los 13 dominios de la taxonomía NANDA-I. Los diagnósticos de enfermería más frecuentes fueron: riesgo de caídas (94,8%); síndrome de fragilidad del anciano (91.3%) y procesos familiares disfuncionales (90.5%). Conclusión e implicaciones para la práctica el perfil de los diagnósticos de enfermería identificados puede contribuir al aumento de indicadores sensibles a la práctica de enfermería, con la planificación e implementación de medidas de atención dirigidas a las necesidades reales de los ancianos institucionalizados, que impactan en la autonomía, independencia y consecuentemente calidad de vida.

Abstract Objective To identify nursing diagnoses of the NANDA-I Taxonomy in institutionalized older adults. Method A descriptive study, conducted with 116 older adults from a long-term institution, carried out based on the identification of nursing diagnoses of the NANDA-I Taxonomy in an instrument based on the theoretical model of Basic Human Needs and validation by experts with support in Risner's diagnosis reasoning. Results 1,555 nursing diagnosis titles were indicated for the 116 older adults, with a mean of 13.4 diagnoses per individual. After excluding repetitions, 39 different diagnosis titles were obtained. Among them, 26 (66.7%) are titles of real diagnoses, 13 (33.3%) are titles of risk diagnoses and are classified in 8 of the 13 domains of the NANDA-I taxonomy. The most frequent nursing diagnoses were the following: risk for falls (94.8%); frail elderly syndrome (91.3%), and dysfunctional family processes (90.5%). Conclusion and implications for the practice The profile of the nursing diagnoses identified can contribute to the increase of sensitive indicators to the nursing practice, with the planning and implementation of care measures directed to the real needs of institutionalized older adults, impacting on autonomy, independence and, consequently, on quality of life.

Resumen Objetivo identificar diagnósticos de enfermería de la taxonomía NANDA-I en ancianos institucionalizados. Método estudio descriptivo, realizado con 116 personas mayores de una residencia gerontológica, a partir de la identificación de diagnósticos de enfermería de la taxonomía NANDA-I en un instrumento basado en el modelo teórico de Necesidades Humanas Básicas y validado por especialistas con apoyo en razonamiento Diagnóstico de Risner. Resultados se indicaron 1.555 títulos de diagnóstico de enfermería para los 116 p edad adultos mayores, con un promedio de 13.4 diagnósticos por adulto mayor. Excluidas las repeticiones, se obtuvieron 39 títulos de diagnóstico diferentes. Entre ellos, 26 (66.7%) son títulos de diagnósticos reales, 13 (33.3%) son títulos de diagnósticos de riesgo, y se clasifican en 8 de los 13 dominios de la taxonomía NANDA-I. Los diagnósticos de enfermería más frecuentes fueron: riesgo de caídas (94,8%); síndrome de fragilidad del anciano (91.3%) y procesos familiares disfuncionales (90.5%). Conclusión e implicaciones para la práctica el perfil de los diagnósticos de enfermería identificados puede contribuir al aumento de indicadores sensibles a la práctica de enfermería, con la planificación e implementación de medidas de atención dirigidas a las necesidades reales de los ancianos institucionalizados, que impactan en la autonomía, independencia y consecuentemente calidad de vida.

Resumo Objetivo identificar diagnósticos de enfermagem da Taxonomia da NANDA-I em idosos institucionalizados. Método estudo descritivo, conduzido com 116 idosos de uma instituição de longa permanência, realizado a partir da identificação de diagnósticos de enfermagem da Taxonomia da NANDA-I em um instrumento fundamentado no modelo teórico das Necessidades Humanas Básicas e validação por especialistas com suporte no raciocínio diagnóstico de Risner. Resultados identificou-se 1.555 títulos de diagnósticos de enfermagem para os 116 idosos, com uma média de 13,4 diagnósticos por idoso. Após exclusão de repetições foram obtidos 39 títulos distintos de diagnósticos. Entre eles, 26 (66,7%) são títulos de diagnósticos reais, 13 (33,3%) títulos de diagnósticos de risco e estão classificados em 8 dos 13 domínios da taxonomia da NANDA-I. Os diagnósticos de enfermagem mais frequentes foram: risco de quedas (94,8%); síndrome do idoso frágil (91,3%) e processos familiares disfuncionais (90,5%). Conclusão e implicações para a prática o perfil de diagnósticos de enfermagem identificado pode contribuir para o incremento de indicadores sensíveis à prática de enfermagem, com o planejamento e implementação de medidas assistenciais direcionadas as reais necessidades dos idosos institucionalizados impactando na autonomia, independência e consequentemente na qualidade de vida.

Resumo Bartonella é um gênero de bactérias zoonóticas emergentes associadas principalmente a eritrócitos e células endoteliais de mamíferos. Morcegos são reservatórios naturais para uma variedade de patógenos importantes que afetam a saúde humana e animal. Além disso, estudos recentes destacaram o papel dos morcegos e de moscas associadas a morcegos na manutenção de Bartonella. No presente estudo, nenhuma das 29 amostras de DNA obtidas a partir do sangue de cinco espécies de morcegos amostrados na ilha de São Luís, estado do Maranhão, Nordeste do Brasil, foi positiva para Bartonella nos ensaios de qPCR direcionados ao gene nuoG. Por outro lado, três das 15 (20%) amostras de DNA de moscas da família Streblidae foram positivas para Bartonella. Os resultados do BLASTn mostraram que as sequências dos genes gltA e rpoB compartilharam identidade, variando de 97,2% a 100%, com as sequências de Bartonella amplificadas em morcegos ou moscas amostrados na Costa Rica ou Brasil. Tais resultados corroboraram as análises filogenéticas realizadas por Inferência Bayesiana. O presente estudo mostrou a ocorrência de Bartonella em moscas de morcegos, auxiliando a esclarecer a distribuição dos genótipos de Bartonella relacionadas a moscas Streblidae na América do Sul.

Abstract Bartonella is a genus of emerging zoonotic bacteria that are mainly associated with mammalian erythrocytes and endothelial cells. Bats are natural reservoirs for a variety of important pathogens that impact human and animal health. Recent reports have highlighted the role of bats and bat flies in the maintenance of Bartonella. Here, we showed that none of the 29 bat DNA blood samples obtained from five bat species in São Luís Island, state of Maranhão, northeastern Brazil, were positive for Bartonella in qPCR assays targeting nuoG. On the other hand, three out of 15 DNA samples (20%) from flies in the family Streblidae were positive for Bartonella. The BLASTn results showed that the gltA and rpoB sequences shared identities ranging from 97.2% to 100%, with Bartonella sequences amplified from bats or bat flies from Costa Rica and Brazil. These findings were supported by phylogenetic analyses based on Bayesian inferences. The present study showed that Bartonella genotypes are present in bat flies, thus shedding some light on the distribution of bat fly-related Bartonella genotypes in South America.

Abstract This study evaluated the interference of enamel and coronal dentin thickness and ambient light in pulse oximetry interpretation, using SaO2the human finger as a reference. Forty-two intact human permanent mandibular molars were sectioned mesiodistally in the central portion. Buccal and lingual dentin surfaces were drilled, and 4 mm, 3 mm and 2 mm enamel/dentin thicknesses were interposed between finger and device, after which SaO2 levels were measured by finger pulse oximetry. A reference device was designed to align pulse oximeter light-emitting diode, buccal surface of tooth, little finger, lingual surface of tooth and photodiode. Variables were described as means and standard deviation, and the confidence interval was reported. Repeated measures analysis of variance (ANOVA) was used to compare the groups, followed by Bonferroni correction. Student t test for paired samples was used to determine presence of ambient light (α = 0.05). Mean SaO2 level was lower at 4.0 ± 0.2 mm thickness, regardless of presence or absence of ambient light (92.7% and 89.3%). The other thicknesses yielded values of 95.5% and 94.5% at 3.0 ± 0.2 mm, and 96.4% and 96.0% at 2.0 ± 0.2 mm (p < 0.001). There were significant differences between SaO2 values at 4.0 ± 0.2 mm, 3.0 ± 0.2 mm and 2.0 ± 0.2 mm thicknesses, in the presence of ambient light. Mean SaO2 level in positive control was 96.3%, and mean pulse rate was 69.5 bpm in presence of ambient light; in the absence of light, these values were 96% and 70.5 bpm. Enamel and dentin thickness interfere with SaO2, regardless of presence or absence of ambient light. The lowest SaO2 levels were found for the thickest tooth samples.

Abstract BRS A501 CL is the first upland rice cultivar with tolerance to the broad-spectrum herbicide Kifix®. It has a medium-length cycle, high stability of whole grain yield at processing, good disease tolerance and a high grain yield.

Abstract BRS Catiana is an irrigated rice cultivar with a medium cycle and excellent grain quality, suited for cultivation in 17 Brazilian states. It has a high yield potential (15,535 kg ha-1) and a mean yield of 7,253 kg ha-1, wide adaptation and late senescence (stay green trait).